Raymond L. Benza M.D. FACC
University of Alabama
Transplant and Preparation Center
At UAB, we will establish a team to facilitate the rapid collection of lungs removed at transplant from patients with primary (idiopathic) pulmonary arterial hypertension (PPAH) and other pulmonary diseases which can be used as controls for the study of PPAH. To permit comparisons, equivalent tissue will be collected from the same areas of each lung and uniquely labeled as to site/source in the lung. Specifically, endothelial cells and smooth muscle cells will be collected for culture from arteries and separately from veins and transferred directly to the Cell Culture Facility.
Arteries, veins, and supporting tissues will be dissected for molecular analysis at the gene expression level and proteomic analysis. Proteomic analysis also will include obtaining tissues to be fixed in various fixatives for immunohistochemical studies of protein expression and research involving in situ hybridization and real time quantitative PCR.
Supporting lung tissues for studies in molecular biology will be collected and snap frozen at the collection site. These tissues will be transferred for processing and storage to the Tissue Processing Core at UAB.
Jerry Eu, M.D.
Duke University
Transplant and Preparation Center
Our Transplant and Preparation Center (TPC) will combine the expertise and resources of pulmonary hypertension, lung transplant and pathology programs at Duke to support the research efforts of Idiopathic Pulmonary Arterial Hypertension (IPAH) Research Network. In each of the past few years, approximately 3 out of 60 adult patients who underwent lung transplantation at Duke had IPAH. The multidisciplinary members in our TPC will procure explanted lungs and blood samples from IPAH and other patients during future lung transplant operations and prepare these tissues for studies designed to better understand the etiology and pathogenesis of IPAH.
Based on our previous experience in harvesting explanted human lung tissues for research purposes, we have devised a protocol as described in our original application that will maximize the procurement and utilization of explanted lungs. Immediately after a lung is removed from the study patient during surgery, 4 members of our TPC will simultaneously process the lung to yield study materials suitable for various cellular, immunohistochemical, genomic and proteomic experiments.
In particular, a trained technician will dissect 2 to 3 lung segments under microscope to isolate pulmonary arteries as small as 0.1 mm in diameter for proteomic and genomic studies. In addition, our TPC will work closely with other investigators in the IPAH Research Network to meet the special needs of certain research projects that may require unusual lung preparation techniques.
For example, if required we can establish primary endothelial and smooth muscle cell cultures from various segments of pulmonary arteries. These cultured vascular cells from explanted lungs of patients with or without IPAH can be invaluable in helping us better understand the molecular pathways involved in IPAH.
In our capacities as physicians, many of us in the Duke TPC will work closely with potential study patients. To avoid the appearance of conflict of interests, the principal investigator who is responsible for enrolling patients for this project will relinquish his role in selecting candidates for lung transplantation. Moreover, to ensure that the participation in this project is entirely voluntary, he will approach a lung transplant candidate for participation only after this patient has fulfilled all of the requirements for lung transplant and has been placed on the active transplant list.
In summary, our research protocol will maximize explanted lung procurement and utilization for research while avoiding potential conflict of interests. Moreover, our expertise in isolating different segments of pulmonary vasculature for various cellular, genomic and proteomic studies and our flexibility to meet additional needs of the IPAH Research Network will make our TPC an invaluable member of the IPAH Research Network.
Dr. Eu has left the PHBI for a job with the NIH in 2011.
Serpil Erzurum, M.D. and Suzy Comhair, M.D.
Cleveland Clinic Foundation Pulmonary Hypertension Studies Requiring
Human Lung, Blood and Bone Marrow
Transplant and Preparation Center
1.) IPAH Endothelial Cell Abnormalities
IPAH is characterized by impaired regulation of both pulmonary hemodynamics and vascular growth. Our preliminary data show that primary pulmonary artery endothelial cells (PAEC) from IPAH lung have enhanced proliferation, migration and abnormal tube formation in vitro. The signal transducer and activator of transcription (STAT) 3, recently identified as a critical regulator for angiogenesis, is persistently activated in IPAH PAEC, but not in control cells.
We hypothesize that the pathogenesis of IPAH stems from abnormal endothelial cells, which may derive from aberrant endothelial progenitor cells (EPCs), and result in autonomous growth signals resulting in increased proliferation, and deregulated angiogenesis. We plan to quantitate proliferation, migration and tube formation of IPAH PAEC, and levels of circulating and bone-marrow derived EPCs, in comparison to healthy and disease controls.
To identify mechanisms that account for the altered biology of IPAH cells, we focus our investigations on the evaluation of the factors leading to the low nitric oxide (NO) in IPAH, in particular post-translational regulatory pathways of NO synthesis, including factors affecting substrate arginine levels. We propose the network collection of pulmonary artery endothelial cells from explanted lungs of IPAH and donor lungs not used in transplantation, blood for EPC measures, serum and plasma analyses of factors that regulate NO synthesis, and bone marrow for evaluation of endothelial progenitor cells. Our signal transduction studies also encompass evaluation for type II bone morphogenetic protein receptor (BMPR2) mutations [described in (2) below].
Finally, our previous studies showed an inverse correlation of NO to pulmonary artery pressure. So, we are conducting a longitudinal study of IPAH patients to test our hypothesis that determinants of NO synthesis, i.e. arginase, methylarginines and arginine, predict outcomes in IPAH. Measure of IPAH patients’ exhaled NO prior to transplantation is suggested for the network cohort. Our studies aim to determine if there are inherent alterations in IPAH endothelial cells, progenitor cell contribution, and identify the causal mechanisms, which may potentially lead to novel therapies for treatment of IPAH.
2.) Genetic Association Studies in IPAH
Mutations of the bone morphogenetic protein receptor II (BMPR2) gene are now recognized to underlie at least 75% of familial PAH cases. Similar mutations are also identifiable in 10-40% of sporadic idiopathic (IPAH) cases. However, the etiology in the majority of IPAH remains unexplained and is likely a combination of genetic and environmental factors. Genetic association studies offer the ability to delineate some of these risk factors and have greatest power when a large number of samples can be uniformly analyzed. The establishment of the IPAH research network therefore provides a unique opportunity to exploit these techniques.
We propose to collect DNA samples from IPAH patients, together with controls matched for age, sex and ethnic background, in order to conduct a collaborative genome-wide association study. While the main focus of the IPAH network is on patients undergoing lung transplantation, it would be highly advantageous to extend the scope for DNA collection, recruiting as many patients as possible, regardless of treatment regimen and transplant status.
Such an approach would both increase the power of studies conducted in the near term, but also lay the foundations for a longitudinal study that might ultimately identify correlates between genetic status and disease progression.
George Noon, M.D.
Baylor College of Medicine
Transplant and Preparation Center
Patients are referred to The Methodist Hospital and Texas Children's Hospital for lung transplant evaluation. They undergo a thorough physical evaluation and testing, meet with pulmonologists, surgeons, transplant coordinators, and the transplant research coordinator. After their testing is complete, they are presented to the thoracic transplant patient selection committee. If the patient is deemed an acceptable candidate for pulmonary transplant, and has financial and social clearance, the transplant coordinators will list the patient with UNOS (United Network Organ Sharing).
At each institution, the physicians and transplant coordinators have access to the lung transplant list. The patients name and diagnosis are included on the list. All patients on the transplant list with idiopathic pulmonary artery hypertension or primary pulmonary hypertension
will be eligible to participate in the study.
When a suitable organ donor is identified by an organ procurement organization for a patient listed at one of these institutions, a call is placed to the transplant coordinator. The coordinator receives a report on the donor. The coordinator contacts the pulmonologist and the surgeon and a decision is made to accept or reject the donor offer. If the organ is accepted, the transplant coordinator admits the recipient to the implanting hospital and the patient is prepped for surgery.
The transplant coordinator communicates with the harvesting surgeon, the implant surgeon and OR personnel to coordinate the time the recipient goes into the operating room for transplant.
The transplant coordinator will collect patient data that includes results of pre-operative tests, medical history, and demographics. This data will be sent to the DCC. Patient information will be identified by a code and not by name outside of the hospital. The transplant research coordinator will ensure that all procedures run smoothly and the collection of data is correct, prospective and secured in a password-protected database in a locked office.
Patient codes will be issued and used for the collection of all tissues and will be used for transmission of patient data (excluding patient name) using this same patient code to the Data Base Collection Center. Only the transplant research coordinator will have access to codes used to identify individual patients.
The person responsible for collecting the tissues will immediately obtain tissues from the operating room during the explantation procedure. This includes collection of blood and tissue followed by immediate processing of tissue necessary to maintain integrity of histology, mRNA, DNA, proteins and cells. The processed samples will then be shipped to the appropriate and designated processing center via Federal Express.
Our original grant submission detailed the exact manner that we would use for processing the tissues and fluids. However, this group will comply with the primary protocols of the Data Bank and Coordinating Center.
Marlene Rabinovitch, M.D.
Stanford University–UCSF-University of Toronto
Transplant and Preparation Center
Patient Population: Lungs removed at transplantation will be procured in collaboration with UCSF and University of Toronto. Those procured from UCSF will be prepared at Stanford and those from the University of Toronto will be prepared at that center. We anticipate 3-5 lungs a year for IPAH, 5-6 for secondary PAH (Eisenmenger’s), 5-6 for secondary PAH (chronic lung disease) and 5-6 matched non-PAH controls.
Both Stanford and the University of Toronto have extensive databases from which clinical information (75 data points/patient at Stanford) can be retrieved. This will serve both for entry into the DCC as well as for future use by Network Investigators.
Procurement of Tissue for the Various Centers: Lung tissue from the right upper lobe (RUL) will be procured for light and electron microscopy (LM, EM) as well as in situ hybridization and from the right middle lobe (RML) for genomic and proteomic studies. The anterior right lower lobe (RLL) will be prepared for immunohistochemistry and laser capture microscopy and studies of the microvasculature with Dr. Donald McDonald as consultant. A portion of the left upper lobe (LUL) will be used for organ culture and the left lower lobe (LLL) for harvest of cells. In addition, bronchoalveolar lavage fluid and cells as well as plasma and DNA will be frozen for future analyzes.
To prepare tissue for LM, EM and in situ hybridization, the RUL will be fully inflated (20-32 cm H2O) and the PA perfused free of blood. The arterial system is fixed by perfusion at the in vivo pressure with universal fixative (1% glutaraldehyde in 4% formaldehyde) and the airway then fixed by perfusion at the inflation pressure. Fixation is maintained for 24-48h depending on the amount of tissue. Three sections are retained to archive the tissues according to the structural changes observed by Movat pentachrome staining. The rest of the fixed tissue will be sent to the Processing Center and embedded in paraffin for light microscopy and for in situ hybridization, in epon for EM or in lowicryl for immunoEM.
To prepare tissues for immunohistochemistry and laser capture microscopy, RLL tissue will be inflated with air and the PA perfused for 2 min with 1% paraformaldehyde and then immersed in fixative for 1h prior to preparation of cryostat sections in OCT. for the Processing Center. Part of the tissue from the posterior segment of the RML will be frozen in liquid N2 and then at-80°C for DNA, RNA and protein analyses at the Genomic and Proteomic Centers.
For detailed studies of the microcirculation, the tissue is cannulated and then perfused for 5min at the in vivo mean pressure using 1% paraformaldehyde, 0.5% glutaraldehyde in PBS then with PBS for 1min, PBS + 1% BSA for one min, biotinylated lectin (5-10 µg/ml) for 1 min, then PBS+ BSA and then PBS for 1 minute each. The tissue is permeabilized by overnight incubation in PBS containing 0.3% Triton X-100, then incubated with avidin-peroxidase complex for 24h and then reacted with DAB.
We will continue organ culture studies from a small piece of the LUL from which proximal PA and distal tissue with intra-acinar PAs are placed in collagen gels and exposed to agents to study the extent of regression of the lesions observed. The LLL tissue is distributed to the Cell Center.
Keith Wille, M.D.
University of Alabama, Birmingham
Transplant and Preparation Center
At UAB, we have established a team to facilitate the rapid collection of lungs removed at transplant from patients with primary (idiopathic) pulmonary arterial hypertension (PPAH) and other pulmonary diseases which can be used as controls for the study of PPAH. To permit comparisons, equivalent tissue is being collected from the same areas of each lung and uniquely labeled as to site/source in the lung.
Specifically, endothelial cells and smooth muscle cells are collected for culture from arteries and separately from veins and transferred directly to the Cell Culture Facility. Arteries, veins, and supporting tissues are dissected for molecular analysis at the gene expression level and proteomic analysis. Proteomic analysis also includes obtaining tissues to be fixed in various fixatives for immunohistochemical studies of protein expression and research involving in situ hybridization and real time quantitative PCR.
Supporting lung tissues for studies in molecular biology are collected and snap frozen at the collection site. These tissues are transferred for processing and storage to the Tissue Processing Core at UAB.
